Construct: shRNA TRCN0000000531

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001433.x-1750s1c1
DNA Barcode:
CTGGAACAAGACGATGGAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ERN1 (2081)
Gene Description:
endoplasmic reticulum to nucleus signaling 1
Transcript:
RefSeq NM_001433.1 (NON-CURRENT)
Match location:
Position 1750 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000000531)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGGAACAAGACGATGGAGAT
Hairpin Sequence:
5'-CCGG-CTGGAACAAGACGATGGAGAT-CTCGAG-ATCTCCATCGTCTTGTTCCAG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGGAACAAGACGATGGAGATCTCGAGATCTCCATCGTCTTGTTCCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGGAACAAGACGATGGAGATCTCGAGATCTCCATCGTCTTGTTCCAG-3'

Other clones with same target sequence:

(none)