Construct: shRNA TRCN0000000546

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001569.x-889s1c1
DNA Barcode:
CATTGTGGACTTTGCTGGCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IRAK1 (3654)
Gene Description:
interleukin 1 receptor associated kinase 1
Transcript:
RefSeq NM_001569.2 (NON-CURRENT)
Match location:
Position 889 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000000546)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATTGTGGACTTTGCTGGCTA
Hairpin Sequence:
5'-CCGG-CATTGTGGACTTTGCTGGCTA-CTCGAG-TAGCCAGCAAAGTCCACAATG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATTGTGGACTTTGCTGGCTACTCGAGTAGCCAGCAAAGTCCACAATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATTGTGGACTTTGCTGGCTACTCGAGTAGCCAGCAAAGTCCACAATG-3'

Other clones with same target sequence:

(none)