Construct: shRNA TRCN0000000571

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001654.x-390s1c1
DNA Barcode:
GACTCATCAAGGGACGAAAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARAF (369)
Gene Description:
A-Raf proto-oncogene, serine/threonine kinase
Transcript:
RefSeq NM_001654.1 (NON-CURRENT)
Match location:
Position 390 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000000571)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTCATCAAGGGACGAAAGA
Hairpin Sequence:
5'-CCGG-GACTCATCAAGGGACGAAAGA-CTCGAG-TCTTTCGTCCCTTGATGAGTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTCATCAAGGGACGAAAGACTCGAGTCTTTCGTCCCTTGATGAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTCATCAAGGGACGAAAGACTCGAGTCTTTCGTCCCTTGATGAGTC-3'

Other clones with same target sequence:

  1. TRCN0000315027