Construct: shRNA TRCN0000000574

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001699.x-1854s1c1
DNA Barcode:
CGAAAGAAGGAGACCCGTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AXL (558)
Gene Description:
AXL receptor tyrosine kinase
Transcript:
RefSeq NM_001699.3 (NON-CURRENT)
Match location:
Position 1854 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000000574)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAAAGAAGGAGACCCGTTAT
Hairpin Sequence:
5'-CCGG-CGAAAGAAGGAGACCCGTTAT-CTCGAG-ATAACGGGTCTCCTTCTTTCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAAAGAAGGAGACCCGTTATCTCGAGATAACGGGTCTCCTTCTTTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAAAGAAGGAGACCCGTTATCTCGAGATAACGGGTCTCCTTCTTTCG-3'

Other clones with same target sequence:

  1. TRCN0000342412
  2. TRCN0000001039