Construct: shRNA TRCN0000000603

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001894.x-909s1c1
DNA Barcode:
CTCTTACCTACGTCAGCTCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CSNK1E (1454)
Gene Description:
casein kinase 1 epsilon
Transcript:
RefSeq NM_001894.4 (CURRENT)
Match location:
Position 909 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000000603)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCTTACCTACGTCAGCTCTT
Hairpin Sequence:
5'-CCGG-CTCTTACCTACGTCAGCTCTT-CTCGAG-AAGAGCTGACGTAGGTAAGAG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCTTACCTACGTCAGCTCTTCTCGAGAAGAGCTGACGTAGGTAAGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCTTACCTACGTCAGCTCTTCTCGAGAAGAGCTGACGTAGGTAAGAG-3'

Other clones with same target sequence:

  1. TRCN0000220099