Construct: shRNA TRCN0000000611

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001896.x-1619s1c1
DNA Barcode:
GCGGCAGTTACATATTATTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CSNK2A2 (1459)
Gene Description:
casein kinase 2 alpha 2
Transcript:
RefSeq NM_001896.1 (NON-CURRENT)
Match location:
Position 1619 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000000611)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGCAGTTACATATTATTAT
Hairpin Sequence:
5'-CCGG-GCGGCAGTTACATATTATTAT-CTCGAG-ATAATAATATGTAACTGCCGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGCAGTTACATATTATTATCTCGAGATAATAATATGTAACTGCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGCAGTTACATATTATTATCTCGAGATAATAATATGTAACTGCCGC-3'

Other clones with same target sequence:

(none)