Construct: shRNA TRCN0000000661

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003607.x-4535s1c1
DNA Barcode:
CGCTCAGTCTATGTTCCCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDC42BPA (8476)
Gene Description:
CDC42 binding protein kinase alpha
Transcript:
RefSeq NM_003607.2 (NON-CURRENT)
Match location:
Position 4535 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000000661)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTCAGTCTATGTTCCCAAA
Hairpin Sequence:
5'-CCGG-CGCTCAGTCTATGTTCCCAAA-CTCGAG-TTTGGGAACATAGACTGAGCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTCAGTCTATGTTCCCAAACTCGAGTTTGGGAACATAGACTGAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTCAGTCTATGTTCCCAAACTCGAGTTTGGGAACATAGACTGAGCG-3'

Other clones with same target sequence:

  1. TRCN0000001332