Construct: shRNA TRCN0000000679

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003656.x-553s1c1
DNA Barcode:
GCTGGATGCTGTGAAATACCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAMK1 (8536)
Gene Description:
calcium/calmodulin dependent protein kinase I
Transcript:
RefSeq NM_003656.3 (NON-CURRENT)
Match location:
Position 553 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000000679)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGATGCTGTGAAATACCT
Hairpin Sequence:
5'-CCGG-GCTGGATGCTGTGAAATACCT-CTCGAG-AGGTATTTCACAGCATCCAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGATGCTGTGAAATACCTCTCGAGAGGTATTTCACAGCATCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGATGCTGTGAAATACCTCTCGAGAGGTATTTCACAGCATCCAGC-3'

Other clones with same target sequence:

(none)