Construct: shRNA TRCN0000000735

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003957.x-2938s1c1
DNA Barcode:
CAGTGTTATTTATTTGCCGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BRSK2 (9024)
Gene Description:
BR serine/threonine kinase 2
Transcript:
RefSeq NM_003957.1 (NON-CURRENT)
Match location:
Position 2938 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000000735)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGTGTTATTTATTTGCCGTT
Hairpin Sequence:
5'-CCGG-CAGTGTTATTTATTTGCCGTT-CTCGAG-AACGGCAAATAAATAACACTG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGTGTTATTTATTTGCCGTTCTCGAGAACGGCAAATAAATAACACTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGTGTTATTTATTTGCCGTTCTCGAGAACGGCAAATAAATAACACTG-3'

Other clones with same target sequence:

(none)