Construct: shRNA TRCN0000000787

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004304.x-3310s1c1
DNA Barcode:
AGAAGAAGAAATCCGTGTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ALK (238)
Gene Description:
ALK receptor tyrosine kinase
Transcript:
RefSeq NM_004304.3 (NON-CURRENT)
Match location:
Position 3310 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000000787)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAAGAAGAAATCCGTGTGAA
Hairpin Sequence:
5'-CCGG-AGAAGAAGAAATCCGTGTGAA-CTCGAG-TTCACACGGATTTCTTCTTCT-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAAGAAGAAATCCGTGTGAACTCGAGTTCACACGGATTTCTTCTTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAAGAAGAAATCCGTGTGAACTCGAGTTCACACGGATTTCTTCTTCT-3'

Other clones with same target sequence:

(none)