Construct: shRNA TRCN0000000793

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004327.x-2355s1c1
DNA Barcode:
AGAGCCAGAAGCAACAAAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BCR (613)
Gene Description:
BCR activator of RhoGEF and GTPase
Transcript:
RefSeq NM_004327.2 (NON-CURRENT)
Match location:
Position 2355 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000000793)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAGCCAGAAGCAACAAAGAT
Hairpin Sequence:
5'-CCGG-AGAGCCAGAAGCAACAAAGAT-CTCGAG-ATCTTTGTTGCTTCTGGCTCT-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAGCCAGAAGCAACAAAGATCTCGAGATCTTTGTTGCTTCTGGCTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAGCCAGAAGCAACAAAGATCTCGAGATCTTTGTTGCTTCTGGCTCT-3'

Other clones with same target sequence:

  1. TRCN0000342375