Construct: shRNA TRCN0000000794

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004329.x-2565s1c1
DNA Barcode:
CGCCAATCTCATACAAGCCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BMPR1A (657)
Gene Description:
bone morphogenetic protein receptor type 1A
Transcript:
RefSeq NM_004329.1 (NON-CURRENT)
Match location:
Position 2565 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000000794)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCCAATCTCATACAAGCCAT
Hairpin Sequence:
5'-CCGG-CGCCAATCTCATACAAGCCAT-CTCGAG-ATGGCTTGTATGAGATTGGCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCCAATCTCATACAAGCCATCTCGAGATGGCTTGTATGAGATTGGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCCAATCTCATACAAGCCATCTCGAGATGGCTTGTATGAGATTGGCG-3'

Other clones with same target sequence:

(none)