Construct: shRNA TRCN0000000825
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_002314.x-3140s1c1
- DNA Barcode:
- CAGTTGAGCATCTAGGAAGTA
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- LIMK1 (3984)
- Gene Description:
- LIM domain kinase 1
- Transcript:
- RefSeq NM_002314.2 (NON-CURRENT)
- Match location:
- Position 3140 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this shRNA:
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Sequence Information
- Target Sequence:
- CAGTTGAGCATCTAGGAAGTA
- Hairpin Sequence:
- 5'-CCGG-CAGTTGAGCATCTAGGAAGTA-CTCGAG-TACTTCCTAGATGCTCAACTG-TTTTT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCAGTTGAGCATCTAGGAAGTACTCGAGTACTTCCTAGATGCTCAACTGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACAGTTGAGCATCTAGGAAGTACTCGAGTACTTCCTAGATGCTCAACTG-3'