Construct: shRNA TRCN0000000843

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005308.x-1942s1c1
DNA Barcode:
CCACCACATAAACTCAAACCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GRK5 (2869)
Gene Description:
G protein-coupled receptor kinase 5
Transcript:
RefSeq NM_005308.1 (NON-CURRENT)
Match location:
Position 1942 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000000843)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACCACATAAACTCAAACCA
Hairpin Sequence:
5'-CCGG-CCACCACATAAACTCAAACCA-CTCGAG-TGGTTTGAGTTTATGTGGTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACCACATAAACTCAAACCACTCGAGTGGTTTGAGTTTATGTGGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACCACATAAACTCAAACCACTCGAGTGGTTTGAGTTTATGTGGTGG-3'

Other clones with same target sequence:

(none)