Construct: shRNA TRCN0000000861

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006251.x-993s1c1
DNA Barcode:
GTTGCCTACCATCTCATAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRKAA1 (5562)
Gene Description:
protein kinase AMP-activated catalytic subunit alpha 1
Transcript:
RefSeq NM_006251.1 (NON-CURRENT)
Match location:
Position 993 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000000861)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTGCCTACCATCTCATAATA
Hairpin Sequence:
5'-CCGG-GTTGCCTACCATCTCATAATA-CTCGAG-TATTATGAGATGGTAGGCAAC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTGCCTACCATCTCATAATACTCGAGTATTATGAGATGGTAGGCAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTGCCTACCATCTCATAATACTCGAGTATTATGAGATGGTAGGCAAC-3'

Other clones with same target sequence:

(none)