Construct: shRNA TRCN0000000900

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014840.x-2796s1c1
DNA Barcode:
GCAGAGAGAATCAGGTTACTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NUAK1 (9891)
Gene Description:
NUAK family kinase 1
Transcript:
RefSeq NM_014840.1 (NON-CURRENT)
Match location:
Position 2796 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000000900)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAGAGAATCAGGTTACTA
Hairpin Sequence:
5'-CCGG-GCAGAGAGAATCAGGTTACTA-CTCGAG-TAGTAACCTGATTCTCTCTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAGAGAATCAGGTTACTACTCGAGTAGTAACCTGATTCTCTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAGAGAATCAGGTTACTACTCGAGTAGTAACCTGATTCTCTCTGC-3'

Other clones with same target sequence:

(none)