Construct: shRNA TRCN0000000954

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002944.x-294s1c1
DNA Barcode:
GCTGCCTAAAGTCGTGTGTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ROS1 (6098)
Gene Description:
ROS proto-oncogene 1, receptor tyrosine kinase
Transcript:
RefSeq NM_002944.2 (CURRENT)
Match location:
Position 294 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000000954)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCCTAAAGTCGTGTGTAA
Hairpin Sequence:
5'-CCGG-GCTGCCTAAAGTCGTGTGTAA-CTCGAG-TTACACACGACTTTAGGCAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCCTAAAGTCGTGTGTAACTCGAGTTACACACGACTTTAGGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCCTAAAGTCGTGTGTAACTCGAGTTACACACGACTTTAGGCAGC-3'

Other clones with same target sequence:

(none)