Construct: shRNA TRCN0000000963

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003494.x-6841s1c1
DNA Barcode:
GATCAGCTCAGACATATTTCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DYSF (8291)
Gene Description:
dysferlin
Transcript:
RefSeq NM_003494.2 (NON-CURRENT)
Match location:
Position 6841 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000000963)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCAGCTCAGACATATTTCA
Hairpin Sequence:
5'-CCGG-GATCAGCTCAGACATATTTCA-CTCGAG-TGAAATATGTCTGAGCTGATC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCAGCTCAGACATATTTCACTCGAGTGAAATATGTCTGAGCTGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCAGCTCAGACATATTTCACTCGAGTGAAATATGTCTGAGCTGATC-3'

Other clones with same target sequence:

(none)