Construct: shRNA TRCN0000000991

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005923.x-4884s1c1
DNA Barcode:
TGTTTAGGCTTCTGTGTGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP3K5 (4217)
Gene Description:
mitogen-activated protein kinase kinase kinase 5
Transcript:
RefSeq NM_005923.3 (NON-CURRENT)
Match location:
Position 4884 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000000991)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTTTAGGCTTCTGTGTGTTT
Hairpin Sequence:
5'-CCGG-TGTTTAGGCTTCTGTGTGTTT-CTCGAG-AAACACACAGAAGCCTAAACA-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTTTAGGCTTCTGTGTGTTTCTCGAGAAACACACAGAAGCCTAAACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTTTAGGCTTCTGTGTGTTTCTCGAGAAACACACAGAAGCCTAAACA-3'

Other clones with same target sequence:

(none)