Construct: shRNA TRCN0000001144

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006276.x-435s1c1
DNA Barcode:
AGCAGGTTTCTTCGTTTGAGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SRSF7 (6432)
Gene Description:
serine and arginine rich splicing factor 7
Transcript:
RefSeq NM_006276.36 (NON-CURRENT)
Match location:
Position 435 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000001144)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCAGGTTTCTTCGTTTGAGT
Hairpin Sequence:
5'-CCGG-AGCAGGTTTCTTCGTTTGAGT-CTCGAG-ACTCAAACGAAGAAACCTGCT-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCAGGTTTCTTCGTTTGAGTCTCGAGACTCAAACGAAGAAACCTGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCAGGTTTCTTCGTTTGAGTCTCGAGACTCAAACGAAGAAACCTGCT-3'

Other clones with same target sequence:

(none)