Construct: shRNA TRCN0000001162

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007279.x-1213s1c1
DNA Barcode:
CGACGAGGAGTATGAGGAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
U2AF2 (11338)
Gene Description:
U2 small nuclear RNA auxiliary factor 2
Transcript:
RefSeq NM_007279.1 (NON-CURRENT)
Match location:
Position 1213 (CDS)
CRISPR Mechanism
CRISPR

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000001162)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACGAGGAGTATGAGGAGAT
Hairpin Sequence:
5'-CCGG-CGACGAGGAGTATGAGGAGAT-CTCGAG-ATCTCCTCATACTCCTCGTCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACGAGGAGTATGAGGAGATCTCGAGATCTCCTCATACTCCTCGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACGAGGAGTATGAGGAGATCTCGAGATCTCCTCATACTCCTCGTCG-3'

Other clones with same target sequence:

  1. TRCN0000314892