Construct: shRNA TRCN0000001185

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015891.x-1001s1c1
DNA Barcode:
GAACATTTATTGGTCACAGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDC40 (51362)
Gene Description:
cell division cycle 40
Transcript:
RefSeq NM_015891.2 (NON-CURRENT)
Match location:
Position 1001 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000001185)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACATTTATTGGTCACAGTA
Hairpin Sequence:
5'-CCGG-GAACATTTATTGGTCACAGTA-CTCGAG-TACTGTGACCAATAAATGTTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACATTTATTGGTCACAGTACTCGAGTACTGTGACCAATAAATGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACATTTATTGGTCACAGTACTCGAGTACTGTGACCAATAAATGTTC-3'

Other clones with same target sequence:

(none)