Construct: shRNA TRCN0000001241

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005826.x-913s1c1
DNA Barcode:
GACGTTATTCTCTATCATCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HNRNPR (10236)
Gene Description:
heterogeneous nuclear ribonucleoprotein R
Transcript:
RefSeq NM_005826.2 (NON-CURRENT)
Match location:
Position 913 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000001241)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACGTTATTCTCTATCATCAA
Hairpin Sequence:
5'-CCGG-GACGTTATTCTCTATCATCAA-CTCGAG-TTGATGATAGAGAATAACGTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACGTTATTCTCTATCATCAACTCGAGTTGATGATAGAGAATAACGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACGTTATTCTCTATCATCAACTCGAGTTGATGATAGAGAATAACGTC-3'

Other clones with same target sequence:

  1. TRCN0000293488