Construct: shRNA TRCN0000001243

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005968.x-444s1c1
DNA Barcode:
AGAGCCTTCATTACAAACATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HNRNPM (4670)
Gene Description:
heterogeneous nuclear ribonucleoprotein M
Transcript:
RefSeq NM_005968.2 (NON-CURRENT)
Match location:
Position 444 (CDS)
CRISPR Mechanism
CRISPR

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000001243)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAGCCTTCATTACAAACATA
Hairpin Sequence:
5'-CCGG-AGAGCCTTCATTACAAACATA-CTCGAG-TATGTTTGTAATGAAGGCTCT-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAGCCTTCATTACAAACATACTCGAGTATGTTTGTAATGAAGGCTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAGCCTTCATTACAAACATACTCGAGTATGTTTGTAATGAAGGCTCT-3'

Other clones with same target sequence:

  1. TRCN0000314754