Construct: shRNA TRCN0000001248

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006743.x-948s1c1
DNA Barcode:
CCCAATGTACCTATAAGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RBM3 (5935)
Gene Description:
RNA binding motif protein 3
Transcript:
RefSeq NM_006743.2 (NON-CURRENT)
Match location:
Position 948 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000001248)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAATGTACCTATAAGAAAT
Hairpin Sequence:
5'-CCGG-CCCAATGTACCTATAAGAAAT-CTCGAG-ATTTCTTATAGGTACATTGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAATGTACCTATAAGAAATCTCGAGATTTCTTATAGGTACATTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAATGTACCTATAAGAAATCTCGAGATTTCTTATAGGTACATTGGG-3'

Other clones with same target sequence:

  1. TRCN0000273463