Construct: shRNA TRCN0000001281

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018091.x-1360s1c1
DNA Barcode:
CGATTACGCAAGTGTTCAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ELP3 (55140)
Gene Description:
elongator acetyltransferase complex subunit 3
Transcript:
RefSeq NM_018091.3 (NON-CURRENT)
Match location:
Position 1360 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000001281)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGATTACGCAAGTGTTCAGAA
Hairpin Sequence:
5'-CCGG-CGATTACGCAAGTGTTCAGAA-CTCGAG-TTCTGAACACTTGCGTAATCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGATTACGCAAGTGTTCAGAACTCGAGTTCTGAACACTTGCGTAATCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGATTACGCAAGTGTTCAGAACTCGAGTTCTGAACACTTGCGTAATCG-3'

Other clones with same target sequence:

(none)