Construct: shRNA TRCN0000001293

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031370.x-1292s1c1
DNA Barcode:
AGAGTGGTTATGGGAAGGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HNRNPD (3184)
Gene Description:
heterogeneous nuclear ribonucleoprotein D
Transcript:
RefSeq NM_031370.1 (NON-CURRENT)
Match location:
Position 1292 (CDS)
CRISPR Mechanism
CRISPR

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000001293)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAGTGGTTATGGGAAGGTAT
Hairpin Sequence:
5'-CCGG-AGAGTGGTTATGGGAAGGTAT-CTCGAG-ATACCTTCCCATAACCACTCT-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAGTGGTTATGGGAAGGTATCTCGAGATACCTTCCCATAACCACTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAGTGGTTATGGGAAGGTATCTCGAGATACCTTCCCATAACCACTCT-3'

Other clones with same target sequence:

  1. TRCN0000293353