Construct: shRNA TRCN0000001298

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031844.x-1132s1c1
DNA Barcode:
CAGTGCTTCTTCCCTTACAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HNRNPU (3192)
Gene Description:
heterogeneous nuclear ribonucleoprotein U
Transcript:
RefSeq NM_031844.1 (NON-CURRENT)
Match location:
Position 1132 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000001298)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGTGCTTCTTCCCTTACAAT
Hairpin Sequence:
5'-CCGG-CAGTGCTTCTTCCCTTACAAT-CTCGAG-ATTGTAAGGGAAGAAGCACTG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGTGCTTCTTCCCTTACAATCTCGAGATTGTAAGGGAAGAAGCACTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGTGCTTCTTCCCTTACAATCTCGAGATTGTAAGGGAAGAAGCACTG-3'

Other clones with same target sequence:

  1. TRCN0000350256