Construct: shRNA TRCN0000001303

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032361.x-1046s1c1
DNA Barcode:
GTGATGACAAAGACGGCAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
THOC3 (84321)
Gene Description:
THO complex 3
Transcript:
RefSeq NM_032361.1 (NON-CURRENT)
Match location:
Position 1046 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000001303)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGATGACAAAGACGGCAAAT
Hairpin Sequence:
5'-CCGG-GTGATGACAAAGACGGCAAAT-CTCGAG-ATTTGCCGTCTTTGTCATCAC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGATGACAAAGACGGCAAATCTCGAGATTTGCCGTCTTTGTCATCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGATGACAAAGACGGCAAATCTCGAGATTTGCCGTCTTTGTCATCAC-3'

Other clones with same target sequence:

  1. TRCN0000342345