Construct: shRNA TRCN0000001306

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032361.x-577s1c1
DNA Barcode:
GCCAAGACACACCGTTCCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
THOC3 (84321)
Gene Description:
THO complex 3
Transcript:
RefSeq NM_032361.1 (NON-CURRENT)
Match location:
Position 577 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000001306)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAAGACACACCGTTCCAAA
Hairpin Sequence:
5'-CCGG-GCCAAGACACACCGTTCCAAA-CTCGAG-TTTGGAACGGTGTGTCTTGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAAGACACACCGTTCCAAACTCGAGTTTGGAACGGTGTGTCTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAAGACACACCGTTCCAAACTCGAGTTTGGAACGGTGTGTCTTGGC-3'

Other clones with same target sequence:

  1. TRCN0000342282