Construct: shRNA TRCN0000001370

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002730.x-1401s1c1
DNA Barcode:
CAGGAAGCCCAGATAATCAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRKACA (5566)
Gene Description:
protein kinase cAMP-activated catalytic subunit alpha
Transcript:
RefSeq NM_002730.2 (NON-CURRENT)
Match location:
Position 1401 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000001370)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGGAAGCCCAGATAATCAGA
Hairpin Sequence:
5'-CCGG-CAGGAAGCCCAGATAATCAGA-CTCGAG-TCTGATTATCTGGGCTTCCTG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGGAAGCCCAGATAATCAGACTCGAGTCTGATTATCTGGGCTTCCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGGAAGCCCAGATAATCAGACTCGAGTCTGATTATCTGGGCTTCCTG-3'

Other clones with same target sequence:

(none)