Construct: shRNA TRCN0000001376

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002747.x-623s1c1
DNA Barcode:
GAAGGTCGCTGTGAAGAAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAPK4 (5596)
Gene Description:
mitogen-activated protein kinase 4
Transcript:
RefSeq NM_002747.2 (NON-CURRENT)
Match location:
Position 623 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000001376)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGGTCGCTGTGAAGAAGAT
Hairpin Sequence:
5'-CCGG-GAAGGTCGCTGTGAAGAAGAT-CTCGAG-ATCTTCTTCACAGCGACCTTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGGTCGCTGTGAAGAAGATCTCGAGATCTTCTTCACAGCGACCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGGTCGCTGTGAAGAAGATCTCGAGATCTTCTTCACAGCGACCTTC-3'

Other clones with same target sequence:

(none)