Construct: shRNA TRCN0000001381

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002759.x-1409s1c1
DNA Barcode:
GCTGTTGGGATGGATTTGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EIF2AK2 (5610)
Gene Description:
eukaryotic translation initiation factor 2 alpha kinase 2
Transcript:
RefSeq NM_002759.1 (NON-CURRENT)
Match location:
Position 1409 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000001381)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTTGGGATGGATTTGATT
Hairpin Sequence:
5'-CCGG-GCTGTTGGGATGGATTTGATT-CTCGAG-AATCAAATCCATCCCAACAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTTGGGATGGATTTGATTCTCGAGAATCAAATCCATCCCAACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTTGGGATGGATTTGATTCTCGAGAATCAAATCCATCCCAACAGC-3'

Other clones with same target sequence:

(none)