Construct: shRNA TRCN0000001385

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002953.x-564s1c1
DNA Barcode:
GCTCTATCTCATTCTGGACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPS6KA1 (6195)
Gene Description:
ribosomal protein S6 kinase A1
Transcript:
RefSeq NM_002953.2 (NON-CURRENT)
Match location:
Position 564 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000001385)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTATCTCATTCTGGACTT
Hairpin Sequence:
5'-CCGG-GCTCTATCTCATTCTGGACTT-CTCGAG-AAGTCCAGAATGAGATAGAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTATCTCATTCTGGACTTCTCGAGAAGTCCAGAATGAGATAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTATCTCATTCTGGACTTCTCGAGAAGTCCAGAATGAGATAGAGC-3'

Other clones with same target sequence:

(none)