Construct: shRNA TRCN0000001406

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004841.x-1980s1c1
DNA Barcode:
CCTACGCCAATACAACAGCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RASAL2 (9462)
Gene Description:
RAS protein activator like 2
Transcript:
RefSeq NM_004841.2 (NON-CURRENT)
Match location:
Position 1980 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000001406)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTACGCCAATACAACAGCAA
Hairpin Sequence:
5'-CCGG-CCTACGCCAATACAACAGCAA-CTCGAG-TTGCTGTTGTATTGGCGTAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTACGCCAATACAACAGCAACTCGAGTTGCTGTTGTATTGGCGTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTACGCCAATACAACAGCAACTCGAGTTGCTGTTGTATTGGCGTAGG-3'

Other clones with same target sequence:

(none)