Construct: shRNA TRCN0000001416

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005813.x-3052s1c1
DNA Barcode:
CAGGACTATCAGACTTGGCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRKD3 (23683)
Gene Description:
protein kinase D3
Transcript:
RefSeq NM_005813.2 (NON-CURRENT)
Match location:
Position 3052 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000001416)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGGACTATCAGACTTGGCTT
Hairpin Sequence:
5'-CCGG-CAGGACTATCAGACTTGGCTT-CTCGAG-AAGCCAAGTCTGATAGTCCTG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGGACTATCAGACTTGGCTTCTCGAGAAGCCAAGTCTGATAGTCCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGGACTATCAGACTTGGCTTCTCGAGAAGCCAAGTCTGATAGTCCTG-3'

Other clones with same target sequence:

  1. TRCN0000349631