Construct: shRNA TRCN0000001462

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138780.x-1145s1c1
DNA Barcode:
GCCTATCAACTACCAGCCTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SYTL5 (94122)
Gene Description:
synaptotagmin like 5
Transcript:
RefSeq NM_138780.1 (NON-CURRENT)
Match location:
Position 1145 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000001462)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTATCAACTACCAGCCTTA
Hairpin Sequence:
5'-CCGG-GCCTATCAACTACCAGCCTTA-CTCGAG-TAAGGCTGGTAGTTGATAGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTATCAACTACCAGCCTTACTCGAGTAAGGCTGGTAGTTGATAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTATCAACTACCAGCCTTACTCGAGTAAGGCTGGTAGTTGATAGGC-3'

Other clones with same target sequence:

(none)