Construct: shRNA TRCN0000001463

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138780.x-1688s1c1
DNA Barcode:
CACCAGAACAACTCCAAGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SYTL5 (94122)
Gene Description:
synaptotagmin like 5
Transcript:
RefSeq NM_138780.1 (NON-CURRENT)
Match location:
Position 1688 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000001463)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACCAGAACAACTCCAAGGAA
Hairpin Sequence:
5'-CCGG-CACCAGAACAACTCCAAGGAA-CTCGAG-TTCCTTGGAGTTGTTCTGGTG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACCAGAACAACTCCAAGGAACTCGAGTTCCTTGGAGTTGTTCTGGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACCAGAACAACTCCAAGGAACTCGAGTTCCTTGGAGTTGTTCTGGTG-3'

Other clones with same target sequence:

(none)