Construct: shRNA TRCN0000001470

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145162.x-791s1c1
DNA Barcode:
CTGATGTCTGGAGCTTAGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP2K5 (5607)
Gene Description:
mitogen-activated protein kinase kinase 5
Transcript:
RefSeq NM_145162.1 (NON-CURRENT)
Match location:
Position 791 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000001470)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGATGTCTGGAGCTTAGGAA
Hairpin Sequence:
5'-CCGG-CTGATGTCTGGAGCTTAGGAA-CTCGAG-TTCCTAAGCTCCAGACATCAG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGATGTCTGGAGCTTAGGAACTCGAGTTCCTAAGCTCCAGACATCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGATGTCTGGAGCTTAGGAACTCGAGTTCCTAAGCTCCAGACATCAG-3'

Other clones with same target sequence:

  1. TRCN0000320676