Construct: shRNA TRCN0000001471

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152720.x-1996s1c1
DNA Barcode:
GCAGTCCCATAGAACAGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NEK3 (4752)
Gene Description:
NIMA related kinase 3
Transcript:
RefSeq NM_152720.1 (NON-CURRENT)
Match location:
Position 1996 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000001471)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTCCCATAGAACAGAAAT
Hairpin Sequence:
5'-CCGG-GCAGTCCCATAGAACAGAAAT-CTCGAG-ATTTCTGTTCTATGGGACTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTCCCATAGAACAGAAATCTCGAGATTTCTGTTCTATGGGACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTCCCATAGAACAGAAATCTCGAGATTTCTGTTCTATGGGACTGC-3'

Other clones with same target sequence:

(none)