Construct: shRNA TRCN0000001492

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004560.x-2071s1c1
DNA Barcode:
CGACAAGCTGAACGTGAAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ROR2 (4920)
Gene Description:
receptor tyrosine kinase like orphan receptor 2
Transcript:
RefSeq NM_004560.2 (NON-CURRENT)
Match location:
Position 2071 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000001492)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACAAGCTGAACGTGAAGAT
Hairpin Sequence:
5'-CCGG-CGACAAGCTGAACGTGAAGAT-CTCGAG-ATCTTCACGTTCAGCTTGTCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACAAGCTGAACGTGAAGATCTCGAGATCTTCACGTTCAGCTTGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACAAGCTGAACGTGAAGATCTCGAGATCTTCACGTTCAGCTTGTCG-3'

Other clones with same target sequence:

(none)