Construct: shRNA TRCN0000001505

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006226.x-2531s1c1
DNA Barcode:
GCTCAGGAATATCGGTCTTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PLCL1 (5334)
Gene Description:
phospholipase C like 1 (inactive)
Transcript:
RefSeq NM_006226.1 (NON-CURRENT)
Match location:
Position 2531 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000001505)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCAGGAATATCGGTCTTAA
Hairpin Sequence:
5'-CCGG-GCTCAGGAATATCGGTCTTAA-CTCGAG-TTAAGACCGATATTCCTGAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCAGGAATATCGGTCTTAACTCGAGTTAAGACCGATATTCCTGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCAGGAATATCGGTCTTAACTCGAGTTAAGACCGATATTCCTGAGC-3'

Other clones with same target sequence:

(none)