Construct: shRNA TRCN0000001515

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007014.x-173s1c1
DNA Barcode:
CCCAAGGTGCATAATCGTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WWP2 (11060)
Gene Description:
WW domain containing E3 ubiquitin protein ligase 2
Transcript:
RefSeq NM_007014.2 (NON-CURRENT)
Match location:
Position 173 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000001515)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAAGGTGCATAATCGTCAA
Hairpin Sequence:
5'-CCGG-CCCAAGGTGCATAATCGTCAA-CTCGAG-TTGACGATTATGCACCTTGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAAGGTGCATAATCGTCAACTCGAGTTGACGATTATGCACCTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAAGGTGCATAATCGTCAACTCGAGTTGACGATTATGCACCTTGGG-3'

Other clones with same target sequence:

  1. TRCN0000320847