Construct: shRNA TRCN0000001697

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003157.x-2949s1c1
DNA Barcode:
GATTACTGTTTACTGAGCCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NEK4 (6787)
Gene Description:
NIMA related kinase 4
Transcript:
RefSeq NM_003157.1 (NON-CURRENT)
Match location:
Position 2949 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000001697)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATTACTGTTTACTGAGCCTT
Hairpin Sequence:
5'-CCGG-GATTACTGTTTACTGAGCCTT-CTCGAG-AAGGCTCAGTAAACAGTAATC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATTACTGTTTACTGAGCCTTCTCGAGAAGGCTCAGTAAACAGTAATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATTACTGTTTACTGAGCCTTCTCGAGAAGGCTCAGTAAACAGTAATC-3'

Other clones with same target sequence:

(none)