Construct: shRNA TRCN0000001721

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014326.x-759s1c1
DNA Barcode:
GTTACGACTTTGATGAGGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DAPK2 (23604)
Gene Description:
death associated protein kinase 2
Transcript:
RefSeq NM_014326.2 (NON-CURRENT)
Match location:
Position 759 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000001721)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTACGACTTTGATGAGGAAT
Hairpin Sequence:
5'-CCGG-GTTACGACTTTGATGAGGAAT-CTCGAG-ATTCCTCATCAAAGTCGTAAC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTACGACTTTGATGAGGAATCTCGAGATTCCTCATCAAAGTCGTAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTACGACTTTGATGAGGAATCTCGAGATTCCTCATCAAAGTCGTAAC-3'

Other clones with same target sequence:

  1. TRCN0000342401