Construct: shRNA TRCN0000001740

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015184.x-3237s1c1
DNA Barcode:
CAAACGATGAAACTGGAGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PLCL2 (23228)
Gene Description:
phospholipase C like 2
Transcript:
RefSeq NM_015184.2 (NON-CURRENT)
Match location:
Position 3237 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000001740)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAACGATGAAACTGGAGAAT
Hairpin Sequence:
5'-CCGG-CAAACGATGAAACTGGAGAAT-CTCGAG-ATTCTCCAGTTTCATCGTTTG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAACGATGAAACTGGAGAATCTCGAGATTCTCCAGTTTCATCGTTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAACGATGAAACTGGAGAATCTCGAGATTCTCCAGTTTCATCGTTTG-3'

Other clones with same target sequence:

(none)