Construct: shRNA TRCN0000001786

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005906.x-1912s1c1
DNA Barcode:
GATCCACTTAGCACCTCTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAK (4117)
Gene Description:
male germ cell associated kinase
Transcript:
RefSeq NM_005906.3 (NON-CURRENT)
Match location:
Position 1912 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000001786)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCCACTTAGCACCTCTCAA
Hairpin Sequence:
5'-CCGG-GATCCACTTAGCACCTCTCAA-CTCGAG-TTGAGAGGTGCTAAGTGGATC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCCACTTAGCACCTCTCAACTCGAGTTGAGAGGTGCTAAGTGGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCCACTTAGCACCTCTCAACTCGAGTTGAGAGGTGCTAAGTGGATC-3'

Other clones with same target sequence:

(none)