Construct: shRNA TRCN0000001915

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015316.x-1262s1c1
DNA Barcode:
GTCTCTCAGTATTGCCTCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPP1R13B (23368)
Gene Description:
protein phosphatase 1 regulatory subunit 13B
Transcript:
RefSeq NM_015316.1 (NON-CURRENT)
Match location:
Position 1262 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000001915)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCTCTCAGTATTGCCTCAAA
Hairpin Sequence:
5'-CCGG-GTCTCTCAGTATTGCCTCAAA-CTCGAG-TTTGAGGCAATACTGAGAGAC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCTCTCAGTATTGCCTCAAACTCGAGTTTGAGGCAATACTGAGAGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCTCTCAGTATTGCCTCAAACTCGAGTTTGAGGCAATACTGAGAGAC-3'

Other clones with same target sequence:

(none)