Construct: shRNA TRCN0000001920
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_016248.x-6706s1c1
- DNA Barcode:
- GCCTATAAAGATTGTGCAGTA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- AKAP11 (11215)
- Gene Description:
- A-kinase anchoring protein 11
- Transcript:
- RefSeq NM_016248.2 (NON-CURRENT)
- Match location:
- Position 6706 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000001920)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCCTATAAAGATTGTGCAGTA
- Hairpin Sequence:
-
5'-CCGG-GCCTATAAAGATTGTGCAGTA-CTCGAG-TACTGCACAATCTTTATAGGC-TTTTT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCCTATAAAGATTGTGCAGTACTCGAGTACTGCACAATCTTTATAGGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCCTATAAAGATTGTGCAGTACTCGAGTACTGCACAATCTTTATAGGC-3'
Other clones with same target sequence:
(none)
