Construct: shRNA TRCN0000001936

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004783.x-704s1c1
DNA Barcode:
GCTTGGCTGGTAATGGAGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TAOK2 (9344)
Gene Description:
TAO kinase 2
Transcript:
RefSeq NM_004783.1 (NON-CURRENT)
Match location:
Position 704 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000001936)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTGGCTGGTAATGGAGTAT
Hairpin Sequence:
5'-CCGG-GCTTGGCTGGTAATGGAGTAT-CTCGAG-ATACTCCATTACCAGCCAAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTGGCTGGTAATGGAGTATCTCGAGATACTCCATTACCAGCCAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTGGCTGGTAATGGAGTATCTCGAGATACTCCATTACCAGCCAAGC-3'

Other clones with same target sequence:

  1. TRCN0000001444